Hóa chất mTSB broth with Novobiocin (20 mg/L) Merck 1092050500 (Chai nhựa 500 g) DEEP52 https://yeuhangduc.vn/

Appearance (clearness): clear

Appearance (color): yellowish-brown

pH-value (25 °C): 7.2 - 7.6

Growth promotion test in accordance with the current version of DIN EN ISO 11133.: 

Mixed inoculum of E.coli and S.aureus

Additionaly monoculture of S.aureus

Inoculum on reference medium (Escherichia coli ATCC 35150 (O157)): ≤ 100

Inoculum on reference medium (Escherichia coli ATCC 700728(WDCM 00014)): ≤ 100

Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100

Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≥ 10000

Growth (Escherichia coli ATCC 35150 (O157)): > 10 yellowish-brown colonies on CT-SMAC agar

Growth (Escherichia coli ATCC 700728 (WDCM 00014)): > 10 yellowish-brown colonies on CT-SMAC agar

Growth Escherichia coli ATCC 25922 (WDCM 00013): > 10 pink colonies on CT-SMAC agar

Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): total inhibition on TSA

Incubation: mTSB broth: 18 - 24 hours at 41,5 °C, aerobic. Confirmation on CT-SMAC Agar resp. TSA: 24 ± 3 hours at 37 ± 1 °C aerobic